qemu

Install MS-DOS 6.22 on MacOS with QEMU

qemu-img create -f qcow msdos.disk 2G  # create image for virtual harddisk
qemu-system-i386 -hda msdos.disk -m 64 -L . -fda dos622_1.img -boot a

during install when asked for next diskette: Ctrl+Alt+2 to go to qemu shell:

eject floppy0 # or fd0? In case you don't know the name type `info block`.
change floppy0 disk2.img

press Ctrl+Alt+1 to go back to installer and finish setup. after installation you can start qemu with the msdos.disk as follows:

qemu-system-i386 -hda msdos.disk -m 64 -L .